A laser puller (Sutter Instruments Co., Novato, CA) was used to generate electrospray emitter tips (~10 × 25 μm inner-outer diameter) on 75 ID × 360 μm OD bare-fused silica capillary columns (Polymicro Phoenix, AZ). The tips were briefly etched with 100% hydrofluoric acid and plugged with 5 μm, 130 Å pore size Bridged Ethylene Hybrid (BEH) C18 particles (Waters, Milford, MA) using an in-house made pressure injection cell with maximum gas pressure rating of ~1500 psi. Then, the column was packed with 1.7 μm diameter, 130 Å pore size BEH C18 particles (Waters, Milford, MA) using the ultra-high pressure (uHP) column packing station, reaching a maximum pressure of ~20,000 to ~30,000 psi.16 (link)
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Ultra-high Pressure Capillary Column Packing
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Corresponding Organization : University of Notre Dame
Other organizations : Energy Center of Wisconsin, University of Wisconsin–Madison
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Variable analysis
independent variables
- Laser puller used to generate electrospray emitter tips
- Etching of tips with 100% hydrofluoric acid
- Plugging of tips with 5 μm, 130 Å pore size Bridged Ethylene Hybrid (BEH) C18 particles using an in-house made pressure injection cell
- Packing of column with 1.7 μm diameter, 130 Å pore size BEH C18 particles using the ultra-high pressure (uHP) column packing station
- Inner-outer diameter of electrospray emitter tips (~10 × 25 μm)
- 75 ID × 360 μm OD bare-fused silica capillary columns (Polymicro Phoenix, AZ)
- Maximum gas pressure rating of ~1500 psi for the in-house made pressure injection cell
- Maximum pressure of ~20,000 to ~30,000 psi for the ultra-high pressure (uHP) column packing station
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