Cardiac Fibrosis Quantification Protocol

Serial sections from paraformaldehyde (PFA) fixed and optimal cutting temperature (OCT) embedded hearts were stained for human cardiac troponin T (Abcam, ab45932) and alpha actinin (Sigma, A7811) following previously published procedures.^{5 (link)} Masson’s trichrome staining was also performed using a standard protocol.^{5 (link)} The scar sizes of 4 EHM versus 4 control hearts were measured by comparing the scar area with LV area in mid-ventricular short axis slices.

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Qin X., Riegler J., Tiburcy M., Zhao X., Chour T., Ndoye B., Nguyen M., Adams J., Ameen M., Denney TS J.r., Yang P.C., Nguyen P., Zimmermann W.H, & Wu J.C. (2016). Magnetic Resonance Imaging of Cardiac Strain Pattern Following Transplantation of Human Tissue Engineered Heart Muscles. Circulation. Cardiovascular imaging, 9(11), e004731.

Publication 2016

ab45932 A7811

Alpha actinin Axis Hearts Human Paraformaldehyde Scar Troponin t Ventricular

Corresponding Organization :

Other organizations : Auburn University

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Variable analysis

independent variables

Heart type (EHM vs. control)

dependent variables

Scar size (scar area compared to LV area)

control variables

Tissue processing (PFA fixed, OCT embedded)
Staining procedures (human cardiac troponin T, alpha actinin, Masson's trichrome)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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