Quantitative Elemental Mapping of Liver and Spleen Tissues

Tissue sections were cut using the Microm HM330 at 10 µm and put onto Superfrost slides. Laser ablation‐inductively coupled plasma‐MS (LA‐ICP‐MS) was used to quantitate the distribution of iron in liver and spleen in formalin fixed tissue sections using external reference standards.³² (link) Iron (Fe), magnesium (Mg) and zinc (Zn) measurements were performed using the New Wave Research UP213 (Kennelec Scientific, Mitcham, Victoria, Australia) (neodymium‐doped yttrium aluminum garnet (Nd:YAG) laser system with a two‐volume large format cell connected to an Agilent 7500 cs ICP‐MS (Agilent Technologies Inc., Santa Clara, CA, USA) for isotopes ⁵⁶Fe, ²⁴Mg and ⁶⁶Zn. This technique involves vaporising material with a UV laser that is transferred into an ICP‐MS via argon gas.³³ , ³⁴ (link) Consecutive sections at 4 µm were also stained for Perls’ Prussian blue iron stain for iron distribution and structural identification.

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Vadolas J., Ng G.Z., Kysenius K., Crouch P.J., Dames S., Eisermann M., Nualkaew T., Vilcassim S., Schaeper U, & Grigoriadis G. (2021). SLN124, a GalNac‐siRNA targeting transmembrane serine protease 6, in combination with deferiprone therapy reduces ineffective erythropoiesis and hepatic iron‐overload in a mouse model of β‐thalassaemia. British Journal of Haematology, 194(1), 200-210.

Publication 2021

Agilent 7500 cs ICP‐MS

Argon Formalin Iron Isotopes Laser ablation Liver Magnesium Nd yag laser Neodymium Plasma Spleen Tissue Yttrium aluminum garnet Zinc

Corresponding Organization : Hudson Institute of Medical Research

Other organizations : University of Melbourne, Florey Institute of Neuroscience and Mental Health, Silence Therapeutics (Germany), Monash Health

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Variable analysis

independent variables

Laser ablation‐inductively coupled plasma‐MS (LA‐ICP‐MS) technique

dependent variables

Distribution of iron (Fe) in liver and spleen tissues
Concentration of iron (Fe), magnesium (Mg) and zinc (Zn)

control variables

Tissue sections cut at 10 µm and put onto Superfrost slides
Formalin-fixed tissue sections
External reference standards for quantification

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