TEM Visualization of Viral Particles

For transmission electron microscopy (TEM), viral particles from infected alfalfa tissues were partially purified using a protocol developed for Poinsettia mosaic virus [15 ]. For TEM observation of virus-like particles generated via the PVX vector in N. benthamiana plants, samples were processed as described in [6 (link)]. Virus captured on the TEM grids was stained with 1% phosphotungstate (PTA) solution. The grids were examined in a Hitachi H-7700 Electron Microscope at the Electron and Confocal Microscope Unit, Beltsville Agricultural Research Center.

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Nemchinov L.G., François S., Roumagnac P., Ogliastro M., Hammond R.W., Mollov D.S, & Filloux D. (2018). Characterization of alfalfa virus F, a new member of the genus Marafivirus. PLoS ONE, 13(9), e0203477.

Publication 2018

H-7700 Electron Microscope

Alfalfa Confocal microscope Electron Microscope electron Mosaic virus Plants Tissues Transmission electron microscopy Vector Virus Virus particles

Corresponding Organization : Université de Montpellier

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Variable analysis

independent variables

Purification protocol for viral particles from infected alfalfa tissues
Processing of samples for TEM observation of virus-like particles generated via the PVX vector in N. benthamiana plants

dependent variables

Visualization of viral particles and virus-like particles using transmission electron microscopy (TEM)

control variables

Staining of virus captured on TEM grids with 1% phosphotungstate (PTA) solution
Examination of the grids in a Hitachi H-7700 Electron Microscope at the Electron and Confocal Microscope Unit, Beltsville Agricultural Research Center

controls

Positive control: Not specified
Negative control: Not specified

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