The experimental procedures and protocols of this study conform to the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of the University of Mississippi Medical Center (protocol 1283, initial approval 2/2014, reapproved 1/2017).
Studies were performed on male and female Flox HO-1 and adipose-specific HO-1 knockout mice. Flox HO-1 mice are designed to delete exons 3–5 and activate red fluorescent protein (dsRed) upon cre-mediated deletion and maintained on a C57BL/6J genetic background as originally described [22 (link)]. Adiponectin-Cre mice were mice purchased from Jackson Labs (Bar Harbor, ME, USA) and were derived from the originally described colony and bred onto a C57BL/6J background [21 (link)]. Mice which contain both the Adiponectin-Cre and the flox HO-1 alleles are considered knockouts while mice which lack the Adiponectin-Cre and only contain the flox HO-1 allele are considered Flox mice. Mice were housed under standard conditions until 6 weeks of age after which time some mice were switched to a 60% high-fat diet (diet # D12492, Research Diets, Inc., New Brunswick, NJ, USA) other mice were allowed full access to standard laboratory chow. The groups of mice consumed each diet for 30 weeks.
Studies were performed on male and female Flox HO-1 and adipose-specific HO-1 knockout mice. Flox HO-1 mice are designed to delete exons 3–5 and activate red fluorescent protein (dsRed) upon cre-mediated deletion and maintained on a C57BL/6J genetic background as originally described [22 (link)]. Adiponectin-Cre mice were mice purchased from Jackson Labs (Bar Harbor, ME, USA) and were derived from the originally described colony and bred onto a C57BL/6J background [21 (link)]. Mice which contain both the Adiponectin-Cre and the flox HO-1 alleles are considered knockouts while mice which lack the Adiponectin-Cre and only contain the flox HO-1 allele are considered Flox mice. Mice were housed under standard conditions until 6 weeks of age after which time some mice were switched to a 60% high-fat diet (diet # D12492, Research Diets, Inc., New Brunswick, NJ, USA) other mice were allowed full access to standard laboratory chow. The groups of mice consumed each diet for 30 weeks.
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