Isolation of EBV-Infected Cell Subsets

The EBV-infected cells were isolated as described previously (Onozawa et al., 2018 (link)). In brief, the peripheral blood mononuclear cells (PBMCs) from patients were isolated by density gradient centrifugation using Lymphoprep™ (Abbott Diagnostics Technologies AS, Oslo, Norway) and sorted into CD4-, CD8-, or CD56-positive fractions by using antibody-conjugated magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). The primary monocytes were obtained by negative selection assay using Pan Monocyte Isolation Kit (Miltenyi Biotec).

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Ohashi A., Uemura Y., Yoshimori M., Wada N., Imadome K.I., Yudo K., Koyama T., Shimizu N., Nishio M, & Arai A. (2022). The Plasma Level of Interleukin-1β Can Be a Biomarker of Angiopathy in Systemic Chronic Active Epstein–Barr Virus Infection. Frontiers in Microbiology, 13, 874998.

Publication 2022

Lymphoprep antibody-conjugated magnetic beads Pan Monocyte Isolation Kit

Antibody Assay Cells Density gradient centrifugation Diagnostics Isolation Lymphoprep Monocyte Patients Peripheral blood mononuclear cells

Corresponding Organization :

Other organizations : Tokyo Medical and Dental University, St. Marianna University School of Medicine, National Center For Child Health and Development

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Variable analysis

independent variables

Isolation of EBV-infected cells from peripheral blood mononuclear cells (PBMCs) using density gradient centrifugation and antibody-conjugated magnetic beads

dependent variables

Proportion of CD4-, CD8-, or CD56-positive cell fractions

control variables

Lymphoprep™ (density gradient centrifugation)
Antibody-conjugated magnetic beads (Miltenyi Biotec)
Pan Monocyte Isolation Kit (negative selection assay)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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