Immunohistochemical Analysis of Tumor Markers

All steps were conducted as described in the previous study [23 (link)]. All tissue specimens were fixed with 37% formalin and then paraffin-embedded, haematoxylin and eosin were used for staining. For IHC, the antibodies were listed as follows: HMMR (1:300, Proteintech, Wuhan, China), phosphor-AKT (Ser473) (1:100, Service Bio, Wuhan, China), N-cadherin (1:100, Service Bio, Wuhan, China), and Ki-67 (1:100, Service Bio, Wuhan, China). All images were captured by an ECLIPSE Ti microscope system (Nikon, Japan).

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Guo K., Liu C., Shi J., Lai C., Gao Z., Luo J., Li Z., Tang Z., Li K, & Xu K. (2023). HMMR promotes prostate cancer proliferation and metastasis via AURKA/mTORC2/E2F1 positive feedback loop. Cell Death Discovery, 9, 48.

Publication 2023

N-cadherin ECLIPSE Ti microscope system

Antibodies Eosin Formalin Haematoxylin Microscope N cadherin Paraffin Phosphor Tissue

Corresponding Organization : Sun Yat-sen University

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Variable analysis

independent variables

HMMR antibody concentration (1:300)
Phosphor-AKT (Ser473) antibody concentration (1:100)
N-cadherin antibody concentration (1:100)
Ki-67 antibody concentration (1:100)

dependent variables

Immunohistochemical staining patterns for HMMR, phosphor-AKT (Ser473), N-cadherin, and Ki-67

control variables

Tissue fixation method (37% formalin)
Tissue embedding method (paraffin-embedded)
Staining method (haematoxylin and eosin)
Microscope system (ECLIPSE Ti, Nikon, Japan)

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