Exosomal Delivery of T-ALZ01 via Sonication

T-ALZ01 was loaded into exosomes through sonication. T-ALZ01 (0.2 mg) was added to 200 μL of exosomes (~ 1×10⁸ exosomes) and the mixture was sonicated using a Cole-Parmer Ultrasonic Processor (Vernon Hills, IL, USA). The sonication settings used were 500 v, 2 kHz, 20% power, 6 cycles by 4 seconds pulse and 2 seconds pause according to Haney et al. [38 (link)]. The mixture was allowed to cool on ice for 2 minutes, and then the sonication cycle was repeated. The success of the drug loading was analyzed using high performance liquid chromatography (HPLC).

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Richards T., Perron J.C., Patel K., Wurpel J., Reznik S.E, & Schanne F. (2023). Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes. Research Square.

Publication Preprint 2023

Ultrasonic Processor

Drug Exosomes Hplc Pulse Seconds Ultrasonic

Corresponding Organization :

Other organizations : St. John's University

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Variable analysis

independent variables

Amount of T-ALZ01 loaded into exosomes (0.2 mg)

dependent variables

Efficiency of drug loading into exosomes (analyzed using HPLC)

control variables

Volume of exosomes used (200 μL, approximately 1x10^8 exosomes)
Sonication settings (500 v, 2 kHz, 20% power, 6 cycles by 4 seconds pulse and 2 seconds pause)
Cooling time on ice (2 minutes between sonication cycles)

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