Metabolic Profiling of Ddr1 Knockout Mice

Analysis of metabolic parameters was performed in vivo as previously described using the Comprehensive Laboratory Animal Monitoring System (CLAMS; Columbus Instruments, Columbus, OH) [27 (link)]. Energy expenditure, food intake, oxygen consumption (VO₂), carbon dioxide production (VCO₂), respiratory exchange ratio (RER), and locomotor activity were assessed in Ddr1^+/+ and Ddr1^−/− mice fed an HFD for 6 weeks (6wk HFD). Mice were acclimatized in the metabolic chambers for 24 h prior to the start of data collection, followed by a 24-hour period of data collection. Data was categorized as diurnal (light cycle) and nocturnal (dark cycle). Data was analyzed using CLAX Software (Columbus Instruments).