Platelets depletion was performed as described previously with penile vein injection of 1 mg/kg low-endotoxin and azide-free anti-CD41 antibody (anti-CD41, BD Biosciences, San Diego, CA), diluted in 200 μL sterile normal saline (0.9% [wt/vol] sodium chloride). Injection of this mixture reduced the number of circulating platelets to <0.05 × 106 value, 1.0–1.2 × 106 published values (23 (link)).
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