ChIP-qPCR analysis was performed as described previously [72 (link)], with slight modification. Briefly, 5 × 107 cells were fixed with 1% paraformaldehyde, lysed, and sonicated to achieve the majority of DNA fragments with 100–1000 bp. DNA fragments were then enriched by immunoprecipitation with 5 μg H3K9me3 antibody (ab8898, Abcam), 7 μg H3K9Ac antibody (ab4441, Abcam), 5 μg Dnmt3b antibody (ab13604, Abcam), 5 μg H3K9me2 antibody (ab1220, Abcam) or 5 μg H3K27me3 (ab6002, Abcam). The eluted protein:DNA complex was reverse-crosslinked at 65 °C overnight. DNA was recovered after proteinase and RNase A treatment. Real-time PCR was performed to compare the histone modification at the Fst promoter region using primers provided in S5 Table
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