Xeno-free human iPSC and ESC culture

De-identified human iPSC lines YH10, BJ4, and 1323–2 were obtained from Dr Bruce Conklin (Gladstone Institutes, described previously in [27 (link)]). The H1 ESCs were obtained from Dr Paul Tesar (Case Western Reserve University). Human ESCs and iPSCs were used between passages 20–40, maintained in xeno-free conditions in E8 medium on Vitronectin-coated (Life Technologies) plates, and passaged every 3–5 days to prevent differentiation. Unless otherwise specified, reagents were purchased from Gibco.

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Zhang M., Ngo J., Pirozzi F., Sun Y.P, & Wynshaw-Boris A. (2018). Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells. Stem Cell Research & Therapy, 9, 67.

Publication 2018

Vitronectin-coated

Escs Human Ipscs Vitronectin

Corresponding Organization :

Other organizations : First Affiliated Hospital of Zhengzhou University, Case Western Reserve University

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Variable analysis

independent variables

De-identified human iPSC lines YH10, BJ4, and 1323–2
H1 ESCs

dependent variables

Not explicitly mentioned

control variables

Passages of human ESCs and iPSCs (between 20-40)
Xeno-free conditions in E8 medium on Vitronectin-coated (Life Technologies) plates
Passaging every 3-5 days to prevent differentiation

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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