We used a 2-photon imaging system described previously63 . All imaging was conducted using a custom-built 2-photon 8 kHz resonant scanner using large aperture fluorescence collection optics (primary dichroic: 45.0 ×65.0 ×3.0 mm T865lpxrxt Chroma, US; secondary dichroic: 52.0 ×72.0 ×3.0 mm FF560-FDi02-t3 Semrock, US; green emission filter: 50.8 mm FF01-520/70 Semrock, US; red emission filter: 50.8 mm FF01-650/150 Semrock, US; GaAsP PMTs for green and red channels, PMT2101 Thorlabs, US) paired with a Nikon 16x water immersion, 0.8 NA, 3.0 mm working distance objective. Laser power was controlled with a Pockel cell (350-80LA modulator, 320RM 401 driver, Conoptics, US). Image acquisition was controlled through commercial software (ScanImage, Vidriotech, US). jRGECO1a expressing cells were imaged at 1020 nm (Chameleon Ultra II, Coherent, US). All frame scans lasted 1–2 minutes and were acquired at 120-240 Hz (40–60x optical zoom; 64–128 lines/frame, 64–128 pixels/line) from dendrites (oblique and tuft). Dendritic jRGECO1a signals were deconvolved to detect putative events by applying the OASIS software package64 (link),65 (link) using an AR1 model with a pre-computed signal decay constant of 300 ms (Arg = 0.97; event threshold = 0.3). All dendritic calcium analyses were performed on the deconvolved events.
Reagent table can be found as Supplementary Table1 .
Reagent table can be found as Supplementary Table
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