Protein Leakage Assessment After Disinfection

The bicinchoninic acid (BCA) assay was conducted to assess the amount of protein leakage after the disinfection treatment [18 (link)]. After undergoing UV irradiation, exposure to an electric field, and combined disinfection methods, the agar blocks were subjected to elution in 10 mL of PBS. Subsequently, 1 mL of the eluent was transferred into a tube and centrifuged at a speed of 8000 rpm for 5 min. The BCA assay was performed according to the protocol provided with the kit.

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Zhang Y., Liang Y., Pan D., Bai S., Wen D., Tang M., Song H., Guo X, & Han H. (2024). Enhancing Escherichia coli Inactivation: Synergistic Mechanism of Ultraviolet Light and High-Voltage Electric Field. Foods, 13(9), 1343.

Publication 2024

Corresponding Organization : South China University of Technology

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Variable analysis

independent variables

UV irradiation
Exposure to an electric field
Combined disinfection methods

dependent variables

Amount of protein leakage

control variables

Agar blocks
Elution in 10 mL of PBS
Centrifugation at 8000 rpm for 5 min
BCA assay performed according to the kit protocol

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