Microscopic Investigation of Chara australis

Thalli of Chara australis R.Br. used for microscopic investigation were grown as described in Schmoelzer et al. (2011 (link)). Internodal cells were isolated from the main axis with a small pair of scissors and left in artificial freshwater (AFW; 10⁻³ M NaCl, 10⁻⁴ M KCl, 10⁻⁴ M CaCl₂) until use. For visualization of the pH in the external medium along the cell surface, 50 μM phenol red (phenolsulfonphthalein; Sigma-Aldrich, St. Louis, USA) was added to the AFW or to AFW containing 1.5 × 10⁻⁴ M NaHCO₃.

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Eremin A., Bulychev A.A., Kluge C., Harbinson J, & Foissner I. (2019). PH-dependent cell–cell interactions in the green alga Chara. Protoplasma, 256(6), 1737-1751.

Publication 2019

phenol red phenolsulfonphthalein

Axis Cell Chara Microscopic Nacl Nahco3 Phenolsulfonphthalein

Corresponding Organization : University of Salzburg

Other organizations : Otto-von-Guericke University Magdeburg, Lomonosov Moscow State University, Wageningen University & Research

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Variable analysis

independent variables

The presence or absence of NaHCO3 in the artificial freshwater (AFW)

dependent variables

Visualization of the pH in the external medium along the cell surface

control variables

Concentration of NaCl (10^-3 M), KCl (10^-4 M), and CaCl2 (10^-4 M) in the AFW
Concentration of phenol red (50 μM) added to the AFW or AFW containing NaHCO3

controls

Positive control: AFW containing 50 μM phenol red
Negative control: AFW containing 50 μM phenol red and 1.5 × 10^-4 M NaHCO3

Annotations

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