Anthropometric and Biochemical Measurements

The patient's anthropometric and biochemical measurements were taken at admission. The height and weight were measured to calculate the body mass index (BMI) by using the formula weight/height² (kg/m²) [24 (link)] while the patients were barefoot and in light clothing. The blood pressure was measured twice after the patient rested about 10 minutes and calculated the average value. The fasting blood samples were taken for measurement of fasting insulin, fasting plasma glucose, lipid profiles, WBC, and monocyte automatically using a Beckman Coulter AU5800 biochemical analyzer (Brea, CA, USA) [24 (link)]. HOMA-IR was calculated by using the formula fasting plasma glucose multiplied by fasting insulin and then divided by 22.5. MHR was determined by dividing the monocyte counts (in 10⁹/l) by HDL-C (in mmol/l). HbA1c was measured by high-performance liquid chromatography on a Tosoh Automated Glycohemoglobin Analyzer HLC-723G11 (Shunan, Yamaguchi, Japan) [24 (link)].