Bile Acid Profiling by LC-MS/MS

The bile acid profile was detected using liquid chromatography-tandem mass spectrometry (LC–MS/MS) with the API3200MD triple quadrupole mass spectrometer (ABSciex, USA) and Shimadzu series liquid chromatograph (Shimadzu, Japan). The reagent kits were purchased from Shanghai ClinMeta Co., Ltd. The assay comprised 15 components: cholic acid (CA), glycocholic acid (GCA), taurocholic acid (TCA), chenodeoxycholic acid (CDCA), glycochenodeoxycholic acid (GCDCA), taurochenodeoxycholic acid (TCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), taurodeoxycholic acid (TDCA), lithocholic acid (LCA), glycolithocholic acid (GLCA), taurolithocholic acid (TLCA), ursodeoxycholic acid (UDCA), glycoursodeoxycholic acid (GUDCA), and tauroursodeoxycholic acid (TUDCA).

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Xu R., Shen J., Song Y., Lu J., Liu Y., Cao Y., Wang Z, & Zhang J. (2024). Exploration of the application potential of serum multi-biomarker model in colorectal cancer screening. Scientific Reports, 14, 10127.

Publication 2024

API3200MD triple quadrupole mass spectrometer Shimadzu series liquid chromatograph

Corresponding Organization :

Other organizations : Renji Hospital, Shanghai Jiao Tong University

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Variable analysis

independent variables

None specified

dependent variables

Concentrations of the following bile acids detected using LC-MS/MS:
1. Cholic acid (CA)
2. Glycocholic acid (GCA)
3. Taurocholic acid (TCA)
4. Chenodeoxycholic acid (CDCA)
5. Glycochenodeoxycholic acid (GCDCA)
6. Taurochenodeoxycholic acid (TCDCA)
7. Deoxycholic acid (DCA)
8. Glycodeoxycholic acid (GDCA)
9. Taurodeoxycholic acid (TDCA)
10. Lithocholic acid (LCA)
11. Glycolithocholic acid (GLCA)
12. Taurolithocholic acid (TLCA)
13. Ursodeoxycholic acid (UDCA)
14. Glycoursodeoxycholic acid (GUDCA)
15. Tauroursodeoxycholic acid (TUDCA)

control variables

None specified

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