Blood Collection and Analysis in Injury

At 24 hours post injury, blood was collected via cardiac puncture, an aliquot was placed into a microcapillary tube, and read for a complete blood count with differential by Hemavet (Drew Scientific, Dallas, TX). The remaining blood was harvested for serum by centrifugation after clotting. Serum aliquots were used to measure IL-6 by ELISA (BD Biosciences, Franklin Lakes, NJ) or liver transaminase levels using a DRI-CHEM 7000 (HESKA, Loveland, CO) as described previously (13 (link)).

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Chen M.M., O’Halloran E.B., Ippolito J.A, & Kovacs E.J. (2016). Kupffer Cell p38 MAPK Signaling Drives Post Burn Hepatic Damage and Pulmonary Inflammation when Alcohol Intoxication Precedes Burn Injury. Critical care medicine, 44(10), e973-e979.

Publication 2016

Hemavet ELISA DRI-CHEM 7000

Blood Cardiac Centrifugation Complete blood count Elisa Injury Liver Puncture Serum Transaminase

Corresponding Organization : Loyola University Chicago

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Variable analysis

independent variables

Time post injury (24 hours)

dependent variables

Complete blood count with differential
IL-6 levels
Liver transaminase levels

control variables

Cardiac puncture for blood collection
Use of microcapillary tube for complete blood count
Centrifugation of blood to obtain serum
ELISA for IL-6 measurement
DRI-CHEM 7000 for liver transaminase measurement

controls

Positive control: Not specified
Negative control: Not specified

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