Nicotine Modulates Inflammatory Responses

After nicotine treatment, cells were harvested and homogenized to extract proteins for caspase-1 activity assay by using a commercially available kit (Biovision). These data were expressed as the fold change compared with control cells. In addition, the cell supernatant was also collected to measure the IL-1β production by a mouse IL-1β ELISA kit (Bender Medsystems, Burlingame, CA) according to the protocol described by the manufacturer [21 (link), 50 (link)].

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Singh G.B., Kshirasagar N., Patibandla S., Puchchakayala G., Koka S, & Boini K.M. (2019). Nicotine instigates podocyte injury via NLRP3 inflammasomes activation. Aging (Albany NY), 11(24), 12810-12821.

Publication 2019

caspase-1 activity mouse IL-1β ELISA kit

Assay Caspase 1 Cell Elisa Il 1β Mouse Nicotine Proteins

Corresponding Organization :

Other organizations : University of Houston

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Variable analysis

independent variables

Nicotine treatment

dependent variables

Caspase-1 activity
IL-1β production

control variables

Control cells

controls

Positive control: Not specified
Negative control: Control cells

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