Inducing Quiescence in HepG2 Cells

To induce quiescence of HepG2 cells by serum starvation, ~70% confluent plates of HepG2 cells were changed to DMEM without FBS for 1–3 days. To induce quiescence by contact inhibition, cells were allowed to grow until they reached 100% confluency. The cultures were maintained for 2, 7 or 14 days with media change (with serum) every two days. Proliferating controls were included for both serum starvation and contact inhibition experiments.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Occean J.R., Yang N., Sun Y., Dawkins M.S., Munk R., Belair C., Dar S., Anerillas C., Wang L., Shi C., Dunn C., Bernier M., Price N.L., Kim J.S., Cui C.Y., Fan J., Bhattacharyya M., De S., Maragkakis M., deCabo R., Sidoli S, & Sen P. (2023). Gene body DNA hydroxymethylation restricts the magnitude of transcriptional changes during aging. bioRxiv.

Publication Preprint 2023

Cells Contact inhibition Hepg2 cells Serum

Corresponding Organization : National Institute on Aging

Other organizations : Albert Einstein College of Medicine, Yale University

Top 5 similar protocols

Variable analysis

independent variables

Serum starvation
Contact inhibition

dependent variables

Quiescence of HepG2 cells

control variables

Initial cell density (~70% confluency)
Media change (with serum) every two days for contact inhibition experiments

controls

Proliferating controls for both serum starvation and contact inhibition experiments

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!