Immunohistochemical investigations were carried out using indirect method of peroxidase with a primary antibody specific for LC3 (anti-LC3, GB11124, Servicebio, Wuhan servicebio technology CO., LTD), TLR4 (anti-TLR4, GB11519, Servicebio), and matrix metallopeptidase 3 (MMP3, anti-MMP3, GB11131, Servicebio). The protocols of immunohistochemiscal investigation were the same as our previous study (Yu et al., 2023 (link)).
Free full text: Click here