Metastatic lung tissues were embedded in paraffin and sectioned, and then immunofluorescence staining was carried out according to a published protocol.25 (link) Primary Abs against E‐cadherin (1:200; 20874‐1‐AP) and vimentin (1:300; 10366‐1‐AP) were obtained from Proteintech. The DAPI DNA dye was obtained from Beyotime Biotechnology. The secondary Ab was a cy3‐conjugated goat anti‐rabbit IgG (Beyotime Biotechnology). All stained tissues were examined and photographed under a NEXcope (NE900) confocal fluorescence microscope (Ningbo Yongxin Optics Co., Ltd).
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Immunofluorescence Analysis of Metastatic Lung Tissues
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Anti igg
Cadherin
Confocal microscope
Dapi
Embedded paraffin
Fluorescence
Goat
Immunofluorescence
Lung
Optics
Rabbit
Stained tissues
Tissues
Vimentin
Corresponding Organization : Zhejiang Chinese Medical University
Other organizations : Imperial College Healthcare NHS Trust, Hammersmith Hospital
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- Embedding metastatic lung tissues in paraffin
- Sectioning the paraffin-embedded tissues
- Performing immunofluorescence staining according to a published protocol
- Expression levels of E-cadherin and vimentin proteins in the metastatic lung tissues
- Primary antibodies against E-cadherin (1:200; 20874-1-AP) and vimentin (1:300; 10366-1-AP) obtained from Proteintech
- DAPI DNA dye obtained from Beyotime Biotechnology
- Cy3-conjugated goat anti-rabbit IgG secondary antibody obtained from Beyotime Biotechnology
- Examination and photography of stained tissues using a NEXcope (NE900) confocal fluorescence microscope (Ningbo Yongxin Optics Co., Ltd)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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