MUC1-C Expression in Cancer Cell Lines

Human BT-549, A549/KRAS(G12S), and H460/KRAS(Q61H) cells were grown in RPMI1640 medium (ATCC, Manassas, VA, USA). MDA-MB-231 and MDA-MB-468 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Corning, Manassas, VA, USA). BT-20 cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (ATCC). Media were supplanted with 10% heat-inactivated fetal bovine serum (HI-FBS), 100 U/ml penicillin and 100 μg/ml streptomycin. These cells were selected for study based on their comparatively high and low levels of MUC1-C expression (Supplemental Fig. S7). Cells stably expressing MUC1-C were generated as described (60 ). Cells were treated with the MUC1-C inhibitor GO-203 or the control CP-2 peptide (32 (link)). Authentication of cells was performed by short tandem repeat (STR) analysis. Cell were monitored for mycoplasma contamination using the MycoAlert® Mycoplasma Detection Kit (Lonza, Rockland, MA, USA).