Nucleic Acid Extraction Methods

Nucleic acid extraction was performed by accredited means within the collaborating laboratories: i) MagNA Pure Compact (Total Nucleic Acid Extraction Kit, input volume of 200μl or 400μl and an elution volume of 50μl) and MagNA Pure 96 (DNA and Viral NA Small Volume Kit, input volume of 200μl and an elution volume of 50μl) extractors (all from Roche), ii) Nucleomag Vet kit (Macherey-Nagel) or MagAttract 96 cador Pathogen Kit (Qiagen) on King Fisher Flex 96 (Thermo Fisher Scientific): Input volume 200μl and elution volume 100μl, and iii) manual and robotic extraction depending on the origin of the specimen, as described by Slomka et al. [48 (link)].

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Nagy A., Černíková L., Kunteová K., Dirbáková Z., Thomas S.S., Slomka M.J., Dán Á., Varga T., Máté M., Jiřincová H, & Brown I.H. (2021). A universal RT-qPCR assay for “One Health” detection of influenza A viruses. PLoS ONE, 16(1), e0244669.

Publication 2021

MagNA Pure Compact MagNA Pure 96 DNA and Viral NA Small Volume Kit Nucleomag Vet kit MagAttract 96 cador Pathogen Kit King Fisher Flex 96

Cador Nucleic acid Pathogen

Corresponding Organization :

Other organizations : National Institute of Public Health, State Veterinary Administration, Animal and Plant Health Agency

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Variable analysis

independent variables

Extraction method (MagNA Pure Compact, MagNA Pure 96, Nucleomag Vet kit, MagAttract 96 cador Pathogen Kit on King Fisher Flex 96, manual and robotic extraction)
Input volume (200μl or 400μl)
Elution volume (50μl or 100μl)

dependent variables

Not explicitly mentioned

control variables

Accredited means within the collaborating laboratories

controls

No positive or negative controls explicitly mentioned

Annotations

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