ID8 cells, obtained from Dr Katherine Roby (University of Kansas Medical Center, KS), were cultured in DMEM supplemented with 4% fetal calf serum, 100µg/ml penicillin, 100µg/ml streptomycin and ITS (5 µg/ml insulin, 5µg/ml transferrin and 5ng/ml sodium selenite). As ID8 was obtained directly from their original source, separate STR validation was not performed. For cytotoxicity assays, cells were plated onto 24 plates (3x103 cells/well) in triplicate. Survival was assessed by MTT assay (Nutlin-3) or sulphorhodamine B assay (rucaparib) after 72 hours.
Protocol detail
ID8 Cell Culture and Cytotoxicity Assay
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Assay
Cells
Cytotoxicity
Fetal calf serum
Insulin
Nutlin 3
Penicillin
Rucaparib
Sodium selenite
Streptomycin
Sulphorhodamine b
Transferrin
Corresponding Organization : Queen Mary University of London
Other organizations : Cancer Research UK Scotland Institute
Top 5 similar protocols
Protocol cited in 12 other protocols
Variable analysis
independent variables
- Nutlin-3
- Rucaparib
- Cell survival
- Cell culture media (DMEM supplemented with 4% fetal calf serum, 100µg/ml penicillin, 100µg/ml streptomycin and ITS)
- Cell density (3x10^3 cells/well)
- Incubation time (72 hours)
- Positive control not explicitly mentioned
- Negative control not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!