Protocol detail

Generation of Chimeric Antibodies for HA Targeting

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XY102, 6F12, KB2, 9H10, and 29E3 have been previously described (29 (link)– (link)32 (link), 34 (link)). Chimeric IgG and IgA antibodies were generated as previously described (36 (link)). Briefly, the variable regions of the group 1 HA stalk-binding monoclonal antibodies 6F12 and KB2 were cloned into human IgG and IgA backbones (pFUSE; InvivoGen). An identical strategy was used for 29E3, a strain-specific antibody that recognizes the head domain of A/California/04/09 HA.

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Mullarkey C.E., Bailey M.J., Golubeva D.A., Tan G.S., Nachbagauer R., He W., Novakowski K.E., Bowdish D.M., Miller M.S, & Palese P. (2016). Broadly Neutralizing Hemagglutinin Stalk-Specific Antibodies Induce Potent Phagocytosis of Immune Complexes by Neutrophils in an Fc-Dependent Manner. mBio, 7(5), e01624-16.

Publication 2016

pFUSE

Antibody Backbones Chimeric Head Iga antibodies Monoclonal antibodies Stalk Strain

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Variable analysis

independent variables

Monoclonal antibodies 6F12, KB2, and 29E3

dependent variables

Generation of chimeric IgG and IgA antibodies

control variables

Previously described protocols for generating chimeric IgG and IgA antibodies (36 (link))

controls

Positive control: Previously described protocols for generating chimeric IgG and IgA antibodies (36 (link))
Negative control: Not explicitly mentioned

Annotations

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