Embryos were fixed, stained, imaged, and recovered for genotyping as previously described [68] (link). Primary and secondary antibody sources were described previously [3] (link), [50] (link), and also included rabbit anti-EOMES (Abcam), rabbit anti-DAB2 (Santa Cruz Biotech), rabbit anti-LAMA1 (Sigma), and rat anti-PECAM1 (BD Biosciences).
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