Characterization of EPB41L5 Protein Expression

The mouse monoclonal antibody against β-actin was purchased from a commercial source (EMD Millipore). Rabbit polyclonal antibodies against EPB41L5 were established as described previously [17 (link)]. Peroxidase-conjugated donkey antibodies against mouse or rabbit IgGs were purchased from Jackson ImmunoResearch Laboratories, Inc. Immunoblotting analysis was performed as described previously [6 (link)] using ECL Western detection reagents (GE Healthcare).

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Otsuka Y., Sato H., Oikawa T., Onodera Y., Nam J.M., Hashimoto A., Fukunaga K., Hatanaka K.C., Hatanaka Y., Matsuno Y., Fukuda S, & Sabe H. (2016). High expression of EPB41L5, an integral component of the Arf6-driven mesenchymal program, correlates with poor prognosis of squamous cell carcinoma of the tongue. Cell Communication and Signaling : CCS, 14, 28.

Publication 2016

ECL Western detection reagents

Actin Antibodies Donkey Immunoblotting analysis Monoclonal antibody Mouse Peroxidase Rabbit

Corresponding Organization :

Other organizations : Hokkaido University, Hokkaido Information University, Hokkaido University Hospital

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Variable analysis

independent variables

Mouse monoclonal antibody against β-actin
Rabbit polyclonal antibodies against EPB41L5

dependent variables

Immunoblotting analysis

control variables

Peroxidase-conjugated donkey antibodies against mouse or rabbit IgGs

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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