DXA and anthropometric measures were collected using an adaptation of the protocol described by Ng et al. (26 (link)). In the current study, each participant underwent 2 whole-body DXA scans with repositioning on either a Hologic Horizon/A system at UCSF or a Hologic Discovery/A system at PBRC and at UHCC (Hologic Inc.). Results of the 2 whole-body DXA scans were averaged. Participants were scanned according to the manufacturer's guidelines. All DXA scans were centrally analyzed at UHCC by a single certified technologist using Hologic Apex 5.5 software. Output from DXA included regional and whole-body percentage fat mass, lean soft tissue mass, and mean VAT and SAT for L1–L5. DXA cross-calibration phantoms were circulated between all sites, and calibration equations were derived to remove systematic bias in all bone and soft tissue results.