Cytokine levels come from those reported in Nicholson et al. [16 ] and were measured as described by use of a Bio-Plex Human Cytokine Assay (Bio-Rad Laboratories) including interleukin (IL)-1B, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-γ, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1a, MIP-1b, vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF)-bb, basic fibroblast growth factor (bFGF), chemokine (C-C motif) ligand (CCL) 2, CCL3, CCL4, CCL5, C-X-C motif chemokine 10 (also known as IP-10), eotaxin and tumor necrosis factor (TNF)-α.
Caspase and LDH levels were measured as described [15 (link)] using the Caspase-Glo 3/7 Assay (Promega, Madison, WI, USA) and Cytotoxicity Detection Kit (Roche Applied Science, Indianapolis, IN, USA), respectively [15 (link)]. MMP-7 and MPO levels were measured using Human Total MMP-7 and Human Myeloperoxidase Quantikine ELISA Kits (R&D systems Minneapolis, MN, USA).
Caspase and LDH levels were measured as described [15 (link)] using the Caspase-Glo 3/7 Assay (Promega, Madison, WI, USA) and Cytotoxicity Detection Kit (Roche Applied Science, Indianapolis, IN, USA), respectively [15 (link)]. MMP-7 and MPO levels were measured using Human Total MMP-7 and Human Myeloperoxidase Quantikine ELISA Kits (R&D systems Minneapolis, MN, USA).
Free full text: Click here
