Following euthanasia, the abdomen of each fish was opened and the entire intestine was removed. Wet mounts were prepared from each intestine by placing the intestine on a glass slide with a 25 × 60 mm coverslip, and the entire intestine was examined with a compound microscope (Leica DMLB, Wetzlar, Germany) at × 200 to quantify the number of total live worms, mature female worms, and dead worms present. After examination, each intestine was preserved in Dietrich’s fixative, processed for histology, and stained with hematoxylin and eosin using our standard protocol [21 ].
Pathological changes were scored by a pathologist (M.K.) based on examination of tissues from each intestine. Following our previous study [22 (link)], two broad categories, inflammation and hyperplastic changes, were scored in zebrafish intestines. A total histopathology score, which is the sum of the inflammation and hyperplasia scores, was also calculated. A description of our scoring criteria follows.
Pathological changes were scored by a pathologist (M.K.) based on examination of tissues from each intestine. Following our previous study [22 (link)], two broad categories, inflammation and hyperplastic changes, were scored in zebrafish intestines. A total histopathology score, which is the sum of the inflammation and hyperplasia scores, was also calculated. A description of our scoring criteria follows.
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