Satellite cells (SCs) were isolated from hind limb muscles using Satellite Cell Isolation Kit (Miltenyi Biotec; Bergisch Gladbach, Germany) as previously described [40 (link)] with the following modifications. The muscle tissues were digested with 0.2% (w/v) collagenase II (Gibco; Waltham, MA, USA) in 37 °C for 90 min. After isolation of the cells using Satellite Cell Isolation Kit (Miltenyi Biotec; Bergisch Gladbach, Germany), the cells were further purified with anti-integrin α-7 MicroBeads (Miltenyi Biotec; Bergisch Gladbach, Germany) according to the manufacturer’s protocol.
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