Immunofluorescence and Immunoblotting Antibodies

Primary antibodies used for immunofluorescence and immunoblotting were rabbit antiserum to LAP2α (245.2) (Vlcek et al. 2002 (link)), mouse monoclonal anti-LAP2α (15/2) (Vlcek et al. 2002 (link)), mouse monoclonal antibody to LAP2α (hybridoma supernatant) (Alexis Biochemicals, 8C10-1H11), goat polyclonal anti-Lamin A/C (Santa Cruz Biotechnology, N-18), mouse monoclonal anti-Lamin A/C (clone 4C11, provided by E. Ogris, Max F. Perutz Laboratories, Vienna, Austria) (Roblek et al. 2010 (link)), rabbit polyclonal anti-lamin A (Dechat et al. 2007 (link)), and rabbit polyclonal anti-lamin C (kindly provided by R. Goldman, Northwestern University, Chicago, IL) (Kochin et al. 2014 (link)), mouse monoclonal anti-progerin (clone 13A4, provided by E. Ogris) (Alexis Biochemicals), monoclonal mouse anti-myc (provided by E. Ogris) (Alexis Biochemicals), rabbit polyclonal anti-ubiquitin (Cell Signaling, #3933), mouse monoclonal anti-Col12A1 (Santa Cruz Biotechnology, A-11), goat polyclonal anti-Col1A1 (Santa Cruz Biotechnology, D-13), rabbit monoclonal anti-TIMP2 (Cell Signaling), goat polyclonal anti-actin (Santa Cruz Biotechnology, I-19), and mouse monoclonal anti-γ-tubulin (Sigma, GTU-88).

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Vidak S., Kubben N., Dechat T, & Foisner R. (2015). Proliferation of progeria cells is enhanced by lamina-associated polypeptide 2α (LAP2α) through expression of extracellular matrix proteins. Genes & Development, 29(19), 2022-2036.

Publication 2015

mouse monoclonal anti-Lamin A/C rabbit polyclonal anti-ubiquitin goat polyclonal anti-actin mouse monoclonal anti-γ-tubulin

Actin Antibodies Antiserum Clone Goat Hybridoma Immunofluorescence Lamin a Lamin a c Lamin c Monoclonal antibody Mouse Rabbit Timp2 Tubulin Ubiquitin

Corresponding Organization :

Other organizations : Medical University of Vienna, National Cancer Institute, National Institutes of Health

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Variable analysis

independent variables

Antibodies used for immunofluorescence and immunoblotting

dependent variables

Not explicitly mentioned

control variables

Positive controls:
- Rabbit antiserum to LAP2α (245.2) (Vlcek et al. 2002)
- Mouse monoclonal anti-LAP2α (15/2) (Vlcek et al. 2002)
- Mouse monoclonal antibody to LAP2α (hybridoma supernatant) (Alexis Biochemicals, 8C10-1H11)
- Goat polyclonal anti-Lamin A/C (Santa Cruz Biotechnology, N-18)
- Mouse monoclonal anti-Lamin A/C (clone 4C11, provided by E. Ogris, Max F. Perutz Laboratories, Vienna, Austria) (Roblek et al. 2010)
- Rabbit polyclonal anti-lamin A (Dechat et al. 2007)
- Rabbit polyclonal anti-lamin C (kindly provided by R. Goldman, Northwestern University, Chicago, IL) (Kochin et al. 2014)
- Mouse monoclonal anti-progerin (clone 13A4, provided by E. Ogris) (Alexis Biochemicals)
- Monoclonal mouse anti-myc (provided by E. Ogris) (Alexis Biochemicals)
- Rabbit polyclonal anti-ubiquitin (Cell Signaling, #3933)
- Mouse monoclonal anti-Col12A1 (Santa Cruz Biotechnology, A-11)
- Goat polyclonal anti-Col1A1 (Santa Cruz Biotechnology, D-13)
- Rabbit monoclonal anti-TIMP2 (Cell Signaling)
- Goat polyclonal anti-actin (Santa Cruz Biotechnology, I-19)
- Mouse monoclonal anti-γ-tubulin (Sigma, GTU-88)

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