Isolation of Microglia from Neonatal Mice

Brain cells were obtained from new born mice (<2-week-old) using a Neural Tissue Dissociation Kit (P) (Milteny Biotec, Cologne, Germany), treated with CD11b antibody-conjugated microbeads (Milteny Biotec), and then isolated by magnetic cell sorting as CD11b⁺ cells, as previously described^{6 (link)}. The isolated cells were plated on poly-D-Lysine (PDL)-coated 96-well plates (BD Biosciences, CA, USA), and then cultured in DMEM/F12 (Gibco, CA, USA) with 10% fetal bovine serum (FBS; Gibco) containing 100 U/ml of penicillin/streptomycin (Sigma-Aldrich, MO, USA).

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Kita M., Ano Y., Inoue A, & Aoki J. (2019). Identification of P2Y receptors involved in oleamide-suppressing inflammatory responses in murine microglia and human dendritic cells. Scientific Reports, 9, 3135.

Publication 2019

Neural Tissue Dissociation Kit (P) PDL)-coated 96-well plates DMEM/F12 penicillin/streptomycin

Antibody Brain Cd11b Cells Lysine Mice Microbeads Neural tissue New born Penicillin Poly Streptomycin

Corresponding Organization :

Other organizations : Kyowa Kirin (Japan), Tohoku University

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Variable analysis

independent variables

Tissue source: brain cells from newborn (<2-week-old) mice

dependent variables

Not explicitly mentioned

control variables

CD11b antibody-conjugated microbeads used for magnetic cell sorting
Cells plated on poly-D-Lysine (PDL)-coated 96-well plates
Culture medium: DMEM/F12 with 10% fetal bovine serum (FBS) and 100 U/ml of penicillin/streptomycin

controls

Positive control: Not specified
Negative control: Not specified

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