RIP and dilncRNA analysis was carried out in NIH2/4 as previously described16 (link), with minor modifications. Briefly, I-SceI-GR-expressing NIH2/4 cells were transfected with the plasmid encoding for N-protein or with an EV as a control at 24 h before triamcinolone acetonide 0.1 µM (Sigma-Aldrich) administration. IP was performed using 5 μg of anti-N-protein, or 10 μg of anti-53BP1 (Supplementary Table 1), or with normal rabbit IgGs (Cell Signaling) as a mock IP.
Other organizations :
FIRC Institute of Molecular Oncology, International Centre for Genetic Engineering and Biotechnology, Istituti di Ricovero e Cura a Carattere Scientifico, University of Padua, Fondazione IRCCS Istituto Neurologico Carlo Besta, University of Trieste, University of Palermo
IP using 10 μg of anti-53BP1 (Supplementary Table 1) or normal rabbit IgGs (Cell Signaling) as a mock IP
controls
Positive control: IP using 5 μg of anti-N-protein
Negative control: IP using normal rabbit IgGs (Cell Signaling) as a mock IP
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