Fasting and fed glycemia: Blood glucose levels at fasting (assessed after 6-h of fasting, performed between 8:00 and 14:00) and under fed conditions were measured in the sacrifice day from a drop of blood collected by venipuncture in the jugular vein with the aid of the Accu-Chek® Aviva glucometer (Roche, Mannheim, Germany).
Hemoglobin A1C (HbA1c) was measured on the day of sacrifice through a drop of blood placed on an automated analyzer (Siemens, DCA Vantage Analyzer TM, Tarrytown, NY, USA).
Glucose tolerance test (GTT): In the first day of the last treatment week (23), rats were administered intraperitoneally with a glucose bolus of 2 g/kg BW following a 6-h fasting period. Blood glucose levels were quantified through the tail vein before the injection and 15, 30, 45 and 60 min after, using the portable device Accu-Chek® Aviva glucometer (Roche, Mannheim, Germany). The area under the curve (AUC) for the GTT was calculated by using the trapezoidal method, as previously described [23 (link)].
Hemoglobin A1C (HbA1c) was measured on the day of sacrifice through a drop of blood placed on an automated analyzer (Siemens, DCA Vantage Analyzer TM, Tarrytown, NY, USA).
Glucose tolerance test (GTT): In the first day of the last treatment week (23), rats were administered intraperitoneally with a glucose bolus of 2 g/kg BW following a 6-h fasting period. Blood glucose levels were quantified through the tail vein before the injection and 15, 30, 45 and 60 min after, using the portable device Accu-Chek® Aviva glucometer (Roche, Mannheim, Germany). The area under the curve (AUC) for the GTT was calculated by using the trapezoidal method, as previously described [23 (link)].
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