Apoptosis Quantification in CML Progenitors

Apoptosis in bulk and CD34⁺CD38⁻ cells was estimated by flow cytometric measurements of annexin V stained cells using a BD FACSArray Bioanalyzer. CFSE stained cells were incubated with CD34 antibody and 7-amino-actinomycin D (7AAD). Apoptosis was estimated in 7AAD⁻ CML progenitor cells using a BD LSR-II flow cytometer. Apoptotic quiescent or proliferating CML progenitor cells were defined as annexin V positivity in the CD34⁺CFSE^bright or CD34⁺CFSE^dim population. Apoptotic leukemia cells co-cultured with MSCs are defined as annexin V+/CD45⁺ cells. Apoptosis was expressed as specific apoptosis [14 (link)]:

\begin{matrix} \frac{% of apoptosis in treated cells - % of apoptosis in untreated cells}{% of visible  untreated cells} & \times & 100 % \end{matrix}

Free full text: Click here

Carter B.Z., Mak P.Y., Mak D.H., Ruvolo V.R., Schober W., McQueen T., Cortes J., Kantarjian H.M., Champlin R.E., Konopleva M, & Andreeff M. (2015). Synergistic effects of p53 activation via MDM2 inhibition in combination with inhibition of Bcl-2 or Bcr-Abl in CD34+ proliferating and quiescent chronic myeloid leukemia blast crisis cells. Oncotarget, 6(31), 30487-30499.

Publication 2015

BD FACSArray Bioanalyzer LSR-II flow cytometer

7aad Actinomycin Annexin v Antibody Apoptosis Bulk Cells Cfse Flow cytometric Leukemia Progenitor cells

Corresponding Organization :

Other organizations : The University of Texas MD Anderson Cancer Center

Top 5 similar protocols

Variable analysis

independent variables

Treatment of cells

dependent variables

Apoptosis in bulk and CD34+CD38- cells
Apoptosis in 7AAD- CML progenitor cells
Apoptosis in quiescent or proliferating CML progenitor cells
Apoptosis in leukemia cells co-cultured with MSCs

control variables

Untreated cells

controls

Positive control: Annexin V stained cells
Negative control: Not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!