Engineered Cardiac Transcription Factors

All plasmids were constructed as previously described [5] (link) and can be found on Addgene using the following catalog numbers: Troponin T-GCaMP5-Zeo (46027), tetO-Hand2 (46028), tetO-NKX2.5 (46029), tetO-GATA4 (46030), tetO-MEF2C (46031), tetO-TBX5 (46032). Plasmids that were used from Addgene also include: FUdeltaGW-rtTA (19780), psPAX2 (12260), pMD2.G (12259), and PGK-H2B-mCherry (21217). All plasmids were amplified in STBL3 bacteria (Invitrogen) and prepared with Qiagen MidiPrep Kits.

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Ifkovits J.L., Addis R.C., Epstein J.A, & Gearhart J.D. (2014). Inhibition of TGFβ Signaling Increases Direct Conversion of Fibroblasts to Induced Cardiomyocytes. PLoS ONE, 9(2), e89678.

Publication 2014

Troponin T-GCaMP5-Zeo tetO-GATA4 FUdeltaGW-rtTA psPAX2 pMD2.G PGK-H2B-mCherry STBL3 bacteria MidiPrep Kits

Bacteria Plasmids Troponin t

Corresponding Organization :

Other organizations : University of Pennsylvania

Top 5 similar protocols

Variable analysis

independent variables

Troponin T-GCaMP5-Zeo
TetO-Hand2
TetO-NKX2.5
TetO-GATA4
TetO-MEF2C
TetO-TBX5

dependent variables

Not explicitly mentioned

control variables

FUdeltaGW-rtTA
PsPAX2
PMD2.G
PGK-H2B-mCherry

positive controls

Not specified

negative controls

Not specified

Annotations

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