U251 cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and U138 cells were obtained from Cell Lines Service (Cell Lines Service, 300,363, Eppelheim, Germany). Cell lines were authenticated using Multiplex Cell Authentication by Multiplexion (Heidelberg, Germany) as described previously [20 (link)]. The single nucleotide polymorphism profiles matched known profiles or were unique. The generation of MACC1 overexpressing cell lines U138/MACC1 and U251/MACC1 and their respective controls (U138/EV and U251/EV) was previously described [16 ]. For experiments inhibiting the transcriptional target of MACC1, c-MET, we added 500 nM of crizotinib (Active Biochem, Hong Kong, China, A-1031) 24 h prior to the start of the experiments.
All cell lines were cultured in 87% (v/v) low glucose DMEM (1 g/l glucose; Gibco, 31,885–023), supplemented with 10% (v/v) FBS (Gibco, 10,500–064), 2% (v/v) non-essential amino acids (Biochrome, K0293) and 1% (v/v). penicillin/streptomycin (Gibco, 15,140–122).
All cell lines were cultured in 87% (v/v) low glucose DMEM (1 g/l glucose; Gibco, 31,885–023), supplemented with 10% (v/v) FBS (Gibco, 10,500–064), 2% (v/v) non-essential amino acids (Biochrome, K0293) and 1% (v/v). penicillin/streptomycin (Gibco, 15,140–122).
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