Two glioma cell lines (DBTRG05-MG and SNB19) and the melanoma M624 tumor cell line were maintained in RPMI 1640 l-glutamine (2 mM) with antibiotics (penicillin, 100 IU/mL, and streptomycin, 100 µg/mL) and 10% FBS (Life Technologies, Carlsbad, USA). The HLA-A2+ glioma tumor and melanoma cell lines were treated with decitabine (Selleckchem, Houston, USA) at 1 µM as described previously [27 (link)].
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