Quantifying Pancreatic Tissue Changes

Pancreatic tissue specimens from Kras and Kras/Slug mice were stained for amylase, CK19, PCNA, p-MLC2(S19) and p-ERK1/2(T202/Y204). Antigen retrieval was conducted as previously described10 (link)13 (link). Photographs for quantitative comparison were taken using FeinOptic microscope and Jenoptik ProgRes C5 camera. Number of Kras and Kras/Slug mice with no staining (0), less than 5% (1+), 5% to 25% (2+), or more than 25% (3+) of the pancreas with CK19 staining was determined. Relative number of p-ERK1/2(+) and PCNA(+) nuclei in Kras and Kras/Slug mice were quantified.

Free full text: Click here

Ebine K., Chow C.R., DeCant B.T., Hattaway H.Z., Grippo P.J., Kumar K, & Munshi H.G. (2016). Slug inhibits pancreatic cancer initiation by blocking Kras-induced acinar-ductal metaplasia. Scientific Reports, 6, 29133.

Publication 2016

FeinOptic microscope ProgRes C5

Amylase Antigen Ck19 Erk1 Kras Mice Microscope Nuclei Pancreas Pcna Slug Tissue

Corresponding Organization :

Other organizations : Northwestern University, University of Illinois Chicago

Top 5 similar protocols

Variable analysis

independent variables

Genotype (Kras, Kras/Slug)

dependent variables

Percentage of pancreas with CK19 staining (0%, <5%, 5-25%, >25%)
Number of p-ERK1/2(+) nuclei
Number of PCNA(+) nuclei

control variables

Antigen retrieval method (as previously described in references 10 and 13)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!