Evaluating Cytotoxicity and Antiviral Effects of EBS

Human microglial CHME3 cells [65 (link)] were grown in DMEM containing 10% fetal bovine serum. Cytotoxicity of EBS was determined by using lactate dehydrogenase (LDH) assay (Roche). Briefly, cells treated with EBS at the indicated doses for overnight underwent LDH assay. Sample absorbance was determined by using an ELISA reader (molecular device) at 490 nm. For antiviral study, cells in 12-well plates were adsorbed with ZIKV at 0.1 of multiplicity of infection for 2 hours with the indicated doses of EBS, washed thoroughly to remove unbound viruses, then incubated for another 24 hours in the presence or absence of EBS. The antiviral effect of EBS was evaluated by immunofluorescence and plaque-forming assays.

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Simanjuntak Y., Liang J.J., Chen S.Y., Li J.K., Lee Y.L., Wu H.C, & Lin Y.L. (2018). Ebselen alleviates testicular pathology in mice with Zika virus infection and prevents its sexual transmission. PLoS Pathogens, 14(2), e1006854.

Publication 2018

LDH) assay ELISA reader

Antiviral Assays Cells Cytotoxicity Device Elisa Fetal bovine serum Human Immunofluorescence Infection Lactate dehydrogenase Microglial cells Plaque Viruses Zikv

Corresponding Organization : Genomics Research Center, Academia Sinica

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Variable analysis

independent variables

EBS at the indicated doses

dependent variables

Cytotoxicity of EBS determined by lactate dehydrogenase (LDH) assay
Antiviral effect of EBS evaluated by immunofluorescence and plaque-forming assays

control variables

DMEM containing 10% fetal bovine serum as the growth medium for CHME3 cells
Cells adsorbed with ZIKV at 0.1 multiplicity of infection

positive controls

Not explicitly mentioned

negative controls

Cells treated with ZIKV without EBS

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