Isolation and Identification of Phytochemicals

6,8-Dimethoxycoumarin, tomentin, and diosmetin were isolated from L. chinensis by column chromatography and used as reference standards. Their structures were confirmed by nuclear magnetic resonance (NMR) [46 (link),47 ,48 (link)], and their purities by HPLC (≥98.0%). Other reference standards, namely, diosmin (≥98.0%) and linarin (≥98.0%), were purchased from ChemFaces Biochemical Co., Ltd. (Wuhan, China). HPLC-grade water, acetonitrile, and methanol were purchased from Honeywell Burdick and Jackson (Muskegon, MI, USA). Formic acid was from DAEJUNG Chemicals & Metals Co., Ltd. (Siheung-si, Korea).

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Park N.J., Jo B.G., Bong S.K., Park S.A., Lee S., Kim Y.K., Yang M.H, & Kim S.N. (2022). Lobelia chinensis Extract and Its Active Compound, Diosmetin, Improve Atopic Dermatitis by Reinforcing Skin Barrier Function through SPINK5/LEKTI Regulation. International Journal of Molecular Sciences, 23(15), 8687.

Publication 2022

diosmin linarin HPLC-grade water acetonitrile methanol Formic acid

Acetonitrile Chromatography Diosmetin Diosmin Formic acid Hplc Linarin Metals Methanol Nuclear magnetic resonance

Corresponding Organization : Pusan National University

Other organizations : Gachon University, Sookmyung Women's University

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Variable analysis

independent variables

Column chromatography

dependent variables

Isolation of 6,8-Dimethoxycoumarin, tomentin, and diosmetin from L. chinensis
Confirmation of their structures by nuclear magnetic resonance (NMR)
Determination of their purities by HPLC (≥98.0%)

control variables

HPLC-grade water, acetonitrile, and methanol were used
Formic acid was used

positive controls

Diosmin (≥98.0%) and linarin (≥98.0%) were used as reference standards, purchased from ChemFaces Biochemical Co., Ltd. (Wuhan, China)

negative controls

No negative controls were explicitly mentioned.

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