LOS profiles were examined using Tricine gel electrophoresis of cell lysates as previously described (36 (link)), using lysates from cells grown to mid-logarithmic phase. After fractionation on Novex 16% tricine gels (Invitrogen), the gels were stained with Pro-Q Emerald 300 (Invitrogen) for LOS staining, and Coomassie Brilliant Blue for total protein staining for sample quantification normalization (36 (link)).
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LOS Profiling via Tricine Gel Electrophoresis
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Corresponding Organization : Tufts University
Other organizations : Northeastern University, Boston College, Broad Institute
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Variable analysis
independent variables
- Cell lysates from cells grown to mid-logarithmic phase
- LOS profiles examined using Tricine gel electrophoresis
- Cell lysates from cells grown to mid-logarithmic phase
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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