The BRB-seq is a technique for multiplexed RNA-seq [25 (link)] which is able to provide high-quality 3′ transcriptomic data at a low cost (e.g., 10-fold lower than Illumina Truseq Stranded mRNA-seq). The data (fastq files) generated from BRB-seq are multiplexed and asymmetrical paired reads. Read R1 contains a 6-bp sample barcode, while read R2 contains the fragment sequence to align to the reference genome.
Free full text: Click here
