PET/CT Image Analysis Protocol

Human images were analyzed using Mirada XD^™ 3.6.8 (Mirada Medical) and PMOD version 3.402 (PMOD Technologies LLC) while the animal images were analyzed using VivoQuant 2020 (Invicro). Three-dimensional volumes of interest (VOIs) were drawn using the CT as a reference and the PET data extracted as time-activity curves (TACs), which were used to calculate tissue AUCs and represented as AUC_{tissue/plasma} ratios^{7 (link),8 (link)}. PET activity was converted from tissue volume to tissue mass using tissue density from the Hounsfield units (CT). Heatmap overlays were created using AMIRA 5.2.1 (Visage Imaging, Inc.) and AMIDE 1.0.6 (Andreas Loening).

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Jain S., Chen X., Arun B., Meza O.N., Sarhan M., Singh M., Jeon B., Mane K., Shah M., Tucker E., Carroll L., Freundlich J., Peloquin C, & Ivaturi V. (2024). Dynamic PET Reveals Compartmentalized Brain and Lung Tissue Antibiotic Exposures. Research Square.

Publication Preprint 2024

VivoQuant 2020 AMIRA 5.2.1

Corresponding Organization :

Other organizations : Johns Hopkins Medicine, Johns Hopkins University, Rutgers, The State University of New Jersey, Rutgers Sexual and Reproductive Health and Rights, University of Maryland, Baltimore, University of Florida, Florida College

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Variable analysis

independent variables

Analysis method (Mirada XD™ 3.6.8, PMOD version 3.402, VivoQuant 2020)

dependent variables

Time-activity curves (TACs)
Tissue AUCs
AUCtissue/plasma ratios

control variables

CT as a reference for drawing three-dimensional volumes of interest (VOIs)
Tissue density from Hounsfield units (CT) for converting PET activity from tissue volume to tissue mass

controls

No positive or negative controls were explicitly mentioned.

Annotations

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