LV and RV function were assessed by pressure catheter in vivo57 (link). Mice were anesthetized using isoflurane. The right carotid vein was exposed, and a 1.4-Fr pressure catheter (SPR-839; Millar Instruments, Houston, TX) was inserted into the RV while recording in digital form (MPVS PL3508 PowerLab 8/35, ADInstruments) at the acquisition rate of 2 kHz for analysis (LabChart 8 pro, ADInstruments). Next, the left carotid artery was exposed, and a 1.4-Fr pressure catheter was inserted into the LV while recording LV pressure. All values were averaged over five consecutive cardiac cycles during stable phase of the respiratory cycle.
Ito S., Hashimoto H., Yamakawa H., Kusumoto D., Akiba Y., Nakamura T., Momoi M., Komuro J., Katsuki T., Kimura M., Kishino Y., Kashimura S., Kunitomi A., Lachmann M., Shimojima M., Yozu G., Motoda C., Seki T., Yamamoto T., Shinya Y., Hiraide T., Kataoka M., Kawakami T., Suzuki K., Ito K., Yada H., Abe M., Osaka M., Tsuru H., Yoshida M., Sakimura K., Fukumoto Y., Yuzaki M., Fukuda K, & Yuasa S. (2022). The complement C3-complement factor D-C3a receptor signalling axis regulates cardiac remodelling in right ventricular failure. Nature Communications, 13, 5409.
Other organizations :
Keio University Hospital, National Defense Medical College, Niigata University, Tokyo Medical and Dental University, Kurume University
Pressure catheter (1.4-Fr) used for RV and LV pressure measurements
Recording of pressure data in digital form at 2 kHz acquisition rate
Averaging of values over five consecutive cardiac cycles during stable respiratory phase
controls
Positive control: None mentioned
Negative control: None mentioned
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