Plasma miRNA profiling using OpenArray
Corresponding Organization : Trinity College Dublin
Other organizations : Dublin City University, Biomedical Research Networking Center on Neurodegenerative Diseases, Instituto de Salud Carlos III, Fundacion Centro De Investigacion De Enfermedades Neurologicas
Variable analysis
- Small RNA isolation method (Serum/Plasma small RNA kit)
- MiRNA expression levels measured using the OpenArray platform
- RNase free water used to suspend small RNA
- Small RNA pooled from 10 samples for reverse transcription reaction
- Pre-amplification of cDNA
- Automated loading of cDNA onto the OpenArray
- 754 human miRNAs amplified from each sample
- 16 replicates of 4 internal controls (ath-miR159a, RNU48, RNU44, and U6 rRNA)
- Ct values normalized to the global mean (GMN)
- RNU48, RNU44, and U6 rRNA
- Ath-miR159a
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