Bacterial Siderophore Production Assay

As previously described [43 (link), 44 (link)], the universal Chrom Azurol S (CAS) assay was used to examine the bacterial isolates' capacity to produce siderophores. One hundred mL of the Chrome Azurol S- hexadecyltrimethylammonium bromide (CAS-HDTMA) solution was mixed with 900 mL of sterile LB agar medium to prepare the CAS agar plates. The plates were inoculated with the isolates and incubated for 5 days at 28 °C. After incubation, the plates were examined for the production of an orange zone surrounding the bacterial growth.

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Alshaikh S.A., El-banna T., Sonbol F, & Farghali M.H. (2024). Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital. Annals of Clinical Microbiology and Antimicrobials, 23, 20.

Publication 2024

Corresponding Organization : Tanta University

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Variable analysis

independent variables

Bacterial isolates

dependent variables

Siderophore production as indicated by the formation of an orange zone surrounding the bacterial growth

control variables

Volume of the Chrome Azurol S- hexadecyltrimethylammonium bromide (CAS-HDTMA) solution mixed with the sterile LB agar medium to prepare the CAS agar plates
Incubation time (5 days) and temperature (28 °C)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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