Culturing Lactobacillus plantarum for Inoculation

Lactobacillus plantarum (L. plantarum) was grown in MRS broth medium at 37°C without shaking. After 24 h, the final concentration of the bacterial solution was 2 × 10⁹ CFU/mL, which was inoculated into the new MRS Broth medium at the rate of 2% for extended culture. After 24 h of culture in a shaker, centrifuged at 4000 rpm for 10 min, take the bacterial mud for use.

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Lv W., Ma Y., Zhang Y., Wang T., Huang J., He S., Du H, & Guo S. (2023). Effects of Lactobacillus plantarum fermented Shenling Baizhu San on gut microbiota, antioxidant capacity, and intestinal barrier function of yellow-plumed broilers. Frontiers in Veterinary Science, 10, 1103023.

Publication 2023

Bacterial Lactobacillus plantarum

Corresponding Organization : South China Agricultural University

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Variable analysis

independent variables

Temperature (37°C)
Shaking (without shaking, then with shaking)

dependent variables

Final concentration of bacterial solution (2 × 10^9 CFU/mL)
Inoculation rate (2%) into new MRS Broth medium

control variables

Growth medium (MRS broth)
Bacterial strain (Lactobacillus plantarum)

notes

Independent variables not explicitly mentioned.
Dependent variables not explicitly mentioned.
Positive and negative controls not specified.

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